anti relm β  (Novus Biologicals)

Journal: Mucosal Immunology

Article Title: Microbial regulation of intestinal motility provides resistance against helminth infection

doi: 10.1038/s41385-022-00498-8

Figure Lengend Snippet: a – f SPF and antibiotic-treated mice (enrofloxacin for 2 weeks then amoxicillin and clavulanic acid for the rest of the experiment) were infected for 0 to 21 days with 200 Hpb larvae. Intestine were processed for histological staining of ( a ) DCLK1 (tuft cells (*), green staining) ( c ) mucus with PAS (goblet cells, purple staining) or ( e ) RELM-β (goblet cells, brown staining). All scales represent 200 μm. For each staining, representative images of day 0 and 14 post infected are displayed. Quantification of ( b ) DCLK1 + , ( d ) mucus-producing and ( f ) RELM-β + cells are represented as an average of at least five fields of view for each individual. Data were pooled from two independent experiments with one experiment including day 0 and 14 time points and the 2nd experiment including the day 28 time point. Each dot represents and individual animal. Data were analysed using two-way ANOVA. b The number of mice per time point are: (1) for SPF mice D0 = 4, D14 = 3 and D28 = 4; (2) for antibiotic-treated mice D0 = 3, D14 = 4 and D28 = 5. d The number of mice per time point are: (1) for SPF mice D0 = 3, D14 = 4 and D28 = 5; (2) For antibiotic-treated mice D0 = 4, D14 = 4 and D28 = 5. f The number of mice per time point are: (1) for SPF mice D0 = 4, D14 = 3 and D28 = 4; (2) for antibiotic-treated mice D0 = 3, D14 = 4 and D28 = 5. * display statically significant differences between groups and # display statistically significant differences compared to the naïve group (0 days post-infection).

Article Snippet: Sections were stained with primary antibodies, anti-RELM-β antibody (Peprotech 500-P215) or control IgG, followed by incubation with secondary antibody, anti-rabbit IgG biotinylated (Jackson Immunoresearch 711-065-152).

Techniques: Infection, Staining

Journal: Histochemistry and cell biology

Article Title: Choosing the right antibody for Resistin-Like Molecule (RELM/FIZZ) family members

doi: 10.1007/s00418-012-1042-0

Figure Lengend Snippet: Specificity of hRELMβ antibodies in Western blot and immunofluorescence assays. In Western blot assays, hRELMβ antibodies from Acris (A), Adipogen, and Chemicon (B) were each highly specific for hRELMβ when tested against all RELM isoforms, including mouse. (C) Only the goat anti-hRELMβ antibody from Acris exhibited binding to hRELMβ in the immunofluorescence assay. Mouse anti-FLAG antibody from Sigma was used to indicate the inductive expression of fusion protein in HEK 293 cells. Cy2-conjugated donkey anti-goat IgG was used as secondary antibody (C, green)

Article Snippet: hRELMβ , Acris Antibodies Inc. , Peptide (28–41) DSVMDKKIKDVLNS , Goat , X , X.

Techniques: Western Blot, Immunofluorescence, Binding Assay, Expressing